Combined Effects of Selenium and α-Tocopherol on Quality and Fertilising Ability of Spermatozoa in Extended Cock Semen Processed and Inseminated On-Farm

Lawanson, A. A and Sokunbi, O. A and Bankole, T. O and Ewuola, E. O (2020) Combined Effects of Selenium and α-Tocopherol on Quality and Fertilising Ability of Spermatozoa in Extended Cock Semen Processed and Inseminated On-Farm. Journal of Agriculture and Veterinary Science (IOSR-JAVS), 13 (1). pp. 42-49. ISSN 2319-2372

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Abstract

Spermatozoon apoptosis in extended semen is a challenge to artificial insemination in poultry production. Extender supplementation with exogenous antioxidants could mitigate lipid peroxidation and improve semen quality. Therefore, effects of selenium and α-tocopherol on quality and fertilising ability of spermatozoa in extended cock semen were assessed. Semen samples were collected from fifteen Lohmann breeder cocks aged 40 weeks, pooled and divided into five groups. Each group was extended with Ringers Solution (RS) without antioxidant (T1), RS+25μgmL-1 α-tocopherol (T2), RS+25μgmL-1 selenium (T3), RS+12.5μgmL-1 α-tocopherol+12.5μgmL-1 selenium (T4), RS+25μgmL-1 selenium+25μgmL-1 α-tocopherol (T5) were assessed in vitro and in vivo. All treatments were evaluated for Spermatozoa Progressive Motility (SPM), Spermatozoa Liveability (SL), Total Antioxidant Capacity (TAC, mmol/L) and Lipid Peroxidation (LP, MMDA/l06 Spermatozoa) were determined using standard procedures. Seventy-five Lohmann breeder hens aged 40 weeks were divided into five groups and inseminated with each of the treatments under room temperature for the in vivo evaluation. Egg fertility and hatchability were recorded weekly for four weeks. Data were analysed using descriptive statistics, correlation and ANOVA at α0.05. Result showed that the effect of α-tocopherol and selenium combinations were not (p>0.05) different among treatments for SPM, SL and LP up to 6h. Egg fertility was (p<0.05) higher at T1 (86.5±7.3%), T2 (86.0±7.6%) and T3 (82.0±5.0%) than T4 (57.0±7.1%) and T5 (72.3±8.7%), while hatchability of set eggs ranged between 40.0±8.8% (T2) and I. Introduction Poultry semen has been reported to be highly susceptible to rapid deterioration due to its high susceptibility to lipid peroxidation during storage and processing for artificial insemination (AI) (Surai et al 2000). Earlier studies on chicken and turkey discovered that lipid peroxidation of spermatozoa membranes takes place in just a few hours during in vitro storage both at body-like temperatures (37 ᵒC) as well as at 0 ᵒC (Bakst 1993). In poultry reproduction, it is well established that artificial insemination in avian species has relative advantages as compared with natural mating (Penfold et al 2000, Brillard 2003). The susceptibility of poultry spermatozoa to lipid peroxidation has therefore restricted the adoption of stored poultry semen to natural mating in most part of the world as against semen of other livestock such as cattle and swine which can be extended in the laboratory and transported to farm at different locations. According to Agarwal et al (2003), poor spermatozoa quality has been reported to be associated with high reactive oxygen species (ROS) production as consequence of lipid peroxidation in semen. Reactive oxygen species may have useful or damaging effects on spermatozoa depending on the location, time of exposure, concentration and type (Agarwal and Saleh 2002). The increasing demand for AI in the poultry industry emphasizes the need for the distribution of good quality sperm. In order for the poultry industry to take advantage of modern AI techniques, proper storage of poultry semen is necessary. As chicken semen is highly concentrated and is of low volume, the extension of neat semen with proper diluents is required prior to AI and storage. Ringer’s solution has been well established to be useful in culturing biological cells where spermatozoa are no exception. However, most conventional semen extender such as Ringer’s solution does not contain antioxidants in their composition. Antioxidants are the agents, which break the oxidative chain reaction, thereby, reducing the oxidative stress (Kumar and Mahmood 2001). Traber and Atkinson (2007) reported that Tocopherol (α-tocopherol) scavenges peroxyl radical. It functions by maintaining the integrity of long chain polyunsaturated fatty acids in 71.3±10.2% (T3). Fertility positively correlated with hatchability (r=0.95). Selenium inclusion at 25μgmL-1 under room temperature improved hatchability in Lohmann breeder hens.

Item Type:	Article
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mr Taiwo Egbeyemi
Date Deposited:	13 Jul 2020 11:51
Last Modified:	13 Jul 2020 11:51
URI:	http://eprints.federalpolyilaro.edu.ng/id/eprint/1018

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