Lawanson, A. A. and Sokunbi, O. A. and Obi, G. C. (2019) IN VITRO EFFECTS OF SELENIUM AND α-TOCOPHEROL INCLUSION ON SPERMATOZOA QUALITY OF EXTENDED COCK SEMEN STORED UNDER ROOM TEMPERATURE. FEPI-JOPAS, 1 (1). pp. 76-82. ISSN 2714-2531
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Abstract
Cock spermatozoa are highly susceptible to deterioration during storage in vitro, hence, the need to study the effect of α-tocopherol and selenium in Ringer’s solution as cock semen extender. Semen samples were collected from fifteen Lohmann breeder cocks, pooled and divided into nine groups: Ringers Solution (RS) without antioxidant (T1), RS with α-tocopherol at 25 (T2), 50 (T3), 75 (T4) or 100μgm/l (T5) and selenium at 25 (T6), 50 (T7), 75 (T8) or 100μgm/l (T9). All treatments were stored under room temperature (27 – 29 ᵒC) and evaluated every 3hours until motility dropped below 50%. Spermatozoa Motility (SM), Liveability, Total Antioxidant Capacity (TAC, mmol/l) and Lipid Peroxidation (LP, MMDA/l06 Spermatozoa) were determined using standard procedures. Data were analysed using ANOVA at α0.05. Result showed that progressive motility decreased below 50% after 6 hours of storage. α-tocopherol inclusion level at 100μgm/L for SM (91.7%) and liveability (94.5%) at 3h evaluation period were significantly higher than control (81.7%) and T9 (85.2%), respectively. The TAC ranged from 2.0 (T9) to 3.2 (T5), while LP ranged from 0.5 (T1) to 2.8 (T7) at 3 hours. It was concluded that α-tocopherol inclusion at 100μgm/l in Ringers solution improved cock spermatozoa motility and liveability.
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Mr Taiwo Egbeyemi |
| Date Deposited: | 13 Jul 2020 11:47 |
| Last Modified: | 13 Jul 2020 11:47 |
| URI: | http://eprints.federalpolyilaro.edu.ng/id/eprint/1016 |
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