IN-VITRO ANTI-LIPOXYGENASE AND PROTEINASE INHIBITORY POTENTIALS OF AQUEOUS LEAF EXTRACT OF Amaranthus hybridusL

Bankole, O. Olusola and Remi-Esan, I. A and Oyede, R. T (2019) IN-VITRO ANTI-LIPOXYGENASE AND PROTEINASE INHIBITORY POTENTIALS OF AQUEOUS LEAF EXTRACT OF Amaranthus hybridusL. In: 4th National Development Conference of the School of Pure and Applied Science, The Federal Polytechnic Ilaro, Ogun State, 2nd – 5th December, 2019., The Federal Polytechnic Ilaro, Ogun State.

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Abstract

Inflammation is a reaction of cells or tissues to injury, infections, chemical irritants or allergies usually characterized by heat, swelling, redness and loss of function. The objective of the present work is to study the in vitro anti-lipoxygenase and proteinase inhibitory activity of the aqueous leaf extract of Amaranthus hybridus. Aqueous extracts of A. hybriduswas assessed for in vitro anti-lipoxygenase and anti-proteinase activity. Qualititive phytochemical screening and estimation of total amounts of phytochemicals present was carried out as well. The extract showed a dose dependent anti-lipoxygenase and proteinase inhibitory action. A. hybridus extract at 80 μg/ml displayed significant (P≤0.05) proteinase inhibitory action of 28.861±0.020when compared with the standard drug diclofenac 28.115±0.000. At the different concentrations, the extract displayed significant (P≤0.05) anti-lipoxygenase activity when compared with the standard drug. At 200 μg/ml the extract produced significant anti-lipoxygenase action (52.783±0.002) when compared with the standard drug (79.2481±0.000). Phytochemical screening shows the presence of flavonoid, terpenoids, steroids, Saponins, Coumarins, Anthocyanin, phenols, Alkaloids, fixed oil in the extract. Tannin, glycoside, amino acid, triterpenes, phlobatannin were found absent. Quantitative estimation of phytochemicals revealed that Phenol, flavonoid, alkaloid, steroid and terpeonoid were found present in appreciable amounts in the plant extract with values 3.037±0.000, 2.295±0.002, 2.133±0.019, 2.993 ± 0.000 and 1.548 ± 0.001. In conclusion, results revealed that the leaf extract possess anti-lipoxygenase and anti-proteinase potentials, and this could be due to the differences in the composition and concentration of bioactive compounds.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	Mr Taiwo Egbeyemi
Date Deposited:	11 May 2020 20:20
Last Modified:	11 May 2020 20:20
URI:	http://eprints.federalpolyilaro.edu.ng/id/eprint/105

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