Quantification Of Cyromazine And Melamine In Fish And Poultry Feeds By High Perfomance Liquid Chromatography – Diode Array Detection

OYEDEJI, ABDULRASAQ and ODEYEMI, B.A and Azeez, L. (2019) Quantification Of Cyromazine And Melamine In Fish And Poultry Feeds By High Perfomance Liquid Chromatography – Diode Array Detection. Journal of Chemical Society of Nigeria. ISSN O795-2066

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Abstract

This paper reports the quantities of cyromazine and its metabolite melamine used as additives in fish and poultry feeds. The two triazine compounds were assayed in fish and poultry feeds obtained from Ilaro, Ogun State in Nigeria by high-performance liquid chromatography coupled with diode-array detection (HPLC-DAD). Fish and poultry feed samples meant for various sizes of fishes and different types of chickens, respectively produced locally and imported were after sampling pulverized, treated with acetonitrile and adjusted to pH 7 with phosphate buffer prior to extraction with alkaline acetonitrile (acetonitrile: 25% ammonia solution (95/5; v/v)) from solid-phase extraction (SPE) cartridges previously conditioned with methanol and phosphate buffer. The extracts were concentrated to 2 mL in a water bath at 20oC, filtered through a 0.45 µm syringe and separated on a Zorbax eclipse plus C18 column (150 x 4.6 mm, 5 µm) with mobile phases consisting of acidified ultrapure water and acetonitrile in gradient elution mode within a run time of four minutes. The two triazine compounds considered in this study were present at a lower concentration in the feeds than the permissible level, but their combined effects could be a potential health risk. It is concluded that their presence resulted from the widespread use of materials that contain melamine and not from adulteration or misuse.

Item Type:	Article
Subjects:	Q Science > Q Science (General)
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	Mr Taiwo Egbeyemi
Date Deposited:	03 Jun 2020 20:00
Last Modified:	03 Jun 2020 20:00
URI:	http://eprints.federalpolyilaro.edu.ng/id/eprint/353

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