EVALUATION OF INVITRO ANTI-INFLAMMATORY POTENTIAL OF AQUEOUS Acanthus montanus (AHONEKUN) LEAVES EXTRACT

Olasunkanmi, Adedoyin A. and Adebayo, M (2021) EVALUATION OF INVITRO ANTI-INFLAMMATORY POTENTIAL OF AQUEOUS Acanthus montanus (AHONEKUN) LEAVES EXTRACT. International Conference of Sciences, Engineering & Environmental, 6 (17). pp. 119-128. ISSN 0794-9650

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Abstract

Inflammation is a normal response to any noxious stimulus that threatens the host and may vary from localized response to a generalized one This study is aimed at evaluating the invitro antiinflammatory potential of aqueous leaves extract of Acanthus montanus (Family:Acanthaceae). In vitro anti-inflammatory potentials were evaluated using standard experimental protocols such as Inhibition of albumin denaturation, Anti-lipoxygenase activity, Membrane stabilization and Proteinase inhibitory action at different concentrations of the extract with Diclofenac sodium used as the standard drug. Aqueous extract of A.montanus leaves were tested for the presence of alkaloid, flavonoid ,tannin, glycoside, steroids, phenol and phlobatannins.The total phenol and flavonoid content were evaluated as well. The total phenol and flavonoid content were found to be (64.99±0.6 mg of Gallic acid/g of equivalent) and (26.97± 0.5mg of Quarcetin/g of equivalent).The IC50 values of the extract and diclofenac were 224.53μg/ml and 183.1μg/ml for inhibition of albumin denaturation, 137.03μg/ml and 159.62μg/ml for Anti-lipoxygenase activity, 250.2μg/ml and 195.44μg/ml for membrane stabilization and 198μg/ml and 191.4μg/ml for Proteinase Inhibitory Action. However, the extract exhibited a significant (P<0.05) inhibitory activity in protein denaturation with an IC50value of(198μg/ml) compared to the standard drug(191.4μg/ml).The Inhibition of Albumin Denaturation might be the possible mechanism by which the extract elicit its anti-inflammatory effect. The results obtained in the present study indicate that the aqueous extract of Acanthus montanus is a potent source of anti-inflammatory agents and this justified its uses in the treatment of various infections.

Item Type:	Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	Mr. Bolanle Yisau I.
Date Deposited:	14 Jun 2023 04:10
Last Modified:	14 Jun 2023 04:10
URI:	http://eprints.federalpolyilaro.edu.ng/id/eprint/2293

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