GREEN SYNTHESIS AND CHARACTERISATION OF SILVER NANOPARTICLES USING EXTRACT FROM LAWSONIA INERMIS (HENNA) LEAF AND THEIR ANTIBACTERIAL ACTIVITY

Abiaziem, C. V and adebisi, E.U (2019) GREEN SYNTHESIS AND CHARACTERISATION OF SILVER NANOPARTICLES USING EXTRACT FROM LAWSONIA INERMIS (HENNA) LEAF AND THEIR ANTIBACTERIAL ACTIVITY. In: 4th National Conference of the School of Pure and Applied Sciences, Dec. 2019, The Federal Polytechnic, Ilaro.

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Abstract

Nanoparticles compared to bulk materials, exhibit improved characteristics due to their size, distribution and morphology and are widely used in numerous scientific fields. Plant extract from Lawsonia inermis was used for the synthesis of silver nanoparticles which was used as an antibacterial agent. The effect of contact time, temperature and concentration was studied on the extract of Lawsonia inermis. The silver nanoparticle was synthesized using silver nitrate and Lawsonia inermis leaf extract until a colour change was observed indicating a reduction from Ag+ to Ago. The synthesised AgNPs were characterised using uv-visible spectrophotometry. The formation and stability of the reduced AgNPs in the colloidal solution were monitored by uv-visible spectroscopy. The effect of concentration showed that the concentration of 0.001 M was the most suitable for the synthesis of AgNPs. Effect of contact time and temperature revealed that the synthesis of AgNPs required a greater time and a temperature of 40oC. The antibacterial activity of the synthesised AgNPs inhibited the growth of bacterium (E.coli and Staphylococusaureus). The outcome of this study showed that synthesised AgNPs had a positive effect on inhibiting the growth of E.coli and Stapylococcusaureus.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	Q Science > Q Science (General)
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	Mr Taiwo Egbeyemi
Date Deposited:	02 Jun 2020 15:29
Last Modified:	02 Jun 2020 15:29
URI:	http://eprints.federalpolyilaro.edu.ng/id/eprint/329

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