Gas Chromatography-Mass Spectroscopic, high performance liquid chromatographic and In-silico characterization of antimicrobial and antioxidant constituents of Rhus longipes (Engl)

Adedoyin, Olasunkanmi and Oluminde, S and Peter, I (2021) Gas Chromatography-Mass Spectroscopic, high performance liquid chromatographic and In-silico characterization of antimicrobial and antioxidant constituents of Rhus longipes (Engl). Arabian Journal of Chemistry, 15 (103601). pp. 1-12. ISSN 1878-5352

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Abstract

Abstract Rhus longipes is one of those underutilized plant species with inherent values. Therefore, we conducted a phytochemical study and investigated the antioxidant and antimicrobial potentials of the plant extracts. Aqueous and ethanol extract was subjected to phytochemical analysis. Gas chromatography-mass spectroscopy was performed to identify the volatile compounds while high performance liquid chromatography was done to identify the phenolic and flavonoids in the ethanol extract. Bioactivity and molecular docking analysis was also done for the identification of the bioactive constituents. Tannins, flavonoids, phenolics, terpenoids, steroids, alkaloids, and saponins were identified both in the ethanol and in aqueous extracts of R. longipes. The extracts and ascorbic acid exhibited radical inhibition in a concentration dependent manner. The IC50 values; 3.23, 4.13, 70.75 mg/mL (ABTS), 200.82, 103.63, 390.83 mg/mL (DPPH), 10.06, 93.46, 253.26 mg/mL (O2 � ), and 99.77, 109.23, 446.34 mg/mL (NO�) for ascorbic acid, ethanol and aqueous extract respectively showed that ethanol extract exhibited better radical inhibition than the aqueous extract. 4- hydroxybenzoic and 4-hydroxycoumarin were the most abundant phenolics in the extract. The ethanol extract of R. longipes demonstrated broad-spectrum antibacterial activity with inhibition zone of 25.5 mm against S. aureus, 27.5 mm against E. coli, and 20.5 mm against P. aeruginosa. The identified phytochemicals demonstrated inhibitory potentials against bacterial glucosamine

Item Type:	Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	Mr. Bolanle Yisau I.
Date Deposited:	14 Jun 2023 03:29
Last Modified:	14 Jun 2023 03:29
URI:	http://eprints.federalpolyilaro.edu.ng/id/eprint/2283

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