Ewuola, E. O. and Balogun, Sherif Babajide and Lawanson, A. A. (2017) Spermatozoa Survival Rate in Stored Cock Semen Extended With Diluent Fortified With Natural Antioxidant Sources under Room Temperature. Nigerian Journal of Animal Production. pp. 100-106.
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NJAP 2016-Spermatozoa Survival Rate in Stored Cock Semen Extended With Diluent Fortified With Natural Antioxidant Sources under Room Temperature.pdf Download (277kB) |
Abstract
An experiment was conducted in a completely randomized design with five treatments in triplicates. Four extenders were formulated: Lake Extender; Coconut water-Orange juice Extender (CWOE); Egg yolk-Tomato juice Extender (EYTE) and Milk based-Carrot juice Extender (MCE), were used to extend cock semen constituting treatments 2, 3, 4 and 5 respectively, while the control (Treatment 1) was unextended semen. Semen was harvested from 8 proven Isa breeder cocks aged 35-40 weeks, pooled and evaluated. The ejaculate was divided into 5 and each part was extended with the extenders at ratio 1:2 (Semen: Extender). The extended and un-extended semen samples were kept under room temperatures (26.6- 27.40C) with relative humidity of 64.0-74.0% for in vitro assessment at 2 hrs interval until percentage motility was less than 60%. The results showed that the mass activity, sperm motility and percentage livability reduced p<0.05) as the time of storage increased. Mass activities of unextended semen and the one extended with coconut water-orange juice were significantly (P<0.05) higher than that of other treatments at 0 hour period of storage. Sperm motility in treatments 1 (95.0%), 3 (93.3%) and 4 (88.3%) was not significantly (p>0.05) different from one another, but they was significantly (p<0.05) higher than treatments 2 (40.0%) and 5 (71.7%). At 2 hours of storage, the mass activity and percentage sperm motility in treatment 2 were significantly (P<0.05) lower than other treatments, while live sperm cells were significantly lower for Treatment 3 than other treatments. At 4 hours of storage, mass activity and motility were zero in Treatment 2, while Treatment 1 recorded the highest values (66.7% and 93.5% respectively). Percent motility in Treatments 3, 4 and 5 were 55.0, 50.0 and 43.3%, respectively. However, percent live sperm cells were significantly (P<0.05) higher in treatments 2, 4, 5 and 1 than the in treatment 3. Both CWOE and EYTE possessed a good keeping quality for semen storage. The extenders fortified with natural antioxidants can be used for on-farm insemination in poultry within two hours of collection.
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > SF Animal culture |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Mr Taiwo Egbeyemi |
| Date Deposited: | 03 Feb 2021 10:10 |
| Last Modified: | 03 Feb 2021 10:10 |
| URI: | http://eprints.federalpolyilaro.edu.ng/id/eprint/1263 |
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